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agilent: gene expression
home services platforms agilent: gene expression

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Agilent's Dual-Mode Gene Expression Analysis Platform gives researchers the ease of one-colour experimental design and the resolving power of two-colour detection in a single platform. 1-colour: allows for experimental design to change throughout project – good for projects that will take place over a long period of time with an unknown number of samples 2-colour: is a more direct comparison of two samples The NEW Agilent SurePrint G3 8x60K Microarrays provide extended coverage with updated transcriptome databases for mRNA targets. The human and mouse 8x60K arrays also include probes for lincRNAs (long intergenic non-coding RNAs). This new array format offers higher throughput for whole-genome profiling and increased cost savings. In addition, it requires lower input of RNA.

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Researchers wishing to perform a differential gene expression analysis experiment using one of Agilent's 60-mer oligonucleotide microarrays can contract the UHNMAC to perform the microarray experiments.

All Agilent catalog Gene Expression arrays are available in "4-plex" or "8-plex" design format where four or eight copies of the genome are printed onto each slide respectively. Not all arrays need to be used; however, unused arrays cannot be used at a later date so pricing is based on a complete slide being used. For best pricing, experiments should be designed to use in either multiples of 4 or 8 arrays. For other options, please inquire.

Service includes:

• Total RNA Quality Checked with Agilent BioAnalyzer ($10 fee is charged if RNA quality deemed too low quality to proceed with the experiment)
• Labelling of one RNA sample per array with cyanine-3 (1-colour only)
• Labelling of two RNA samples per array with cyanine-3 and cyanine-5 (one control/universal reference and one experimental) (2-colour only)
• Hybridisation/washing/scanning of arrays
• Spot quantification ( Agilent Feature Extraction)
• Feature Extraction output files sent to customer (FTP)

We also offer an optional data analysis service. Please enquire directly for more information.

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Sample Requirement:

High quality total RNA is required for microarray experiments. The quality of the samples is checked upon arrival using the Agilent 2100 Bioanalyzer. An RNA Integrity Number (RIN) of 7.5 or above is required for guaranteed good results. In general, 100 ng of total RNA is required for labeling. However, up to 500 ng can be used for quality and quantity assessment. Please note that if samples do not meet quality control and the customer decides not to continue with the project, a fee of $10/sample will be charged.

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Pricing: Download
Please note that this pricing is for academic groups. Commercial customers should contact us for a quote.

Contact: Agilent Service at agilent@microarrays.ca

Custom designs are also available.

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Gene expression profiling is also available on the Affymetrix, Illumina, and UHNMAC array platforms.